Determination of glutathione peroxidase activity and oxidized/reduced glutathione ratio in patients with colon cancer

Colon cancer (CC) is accepted as the third type of that causes death in world. Oxidative stress and low glutathione peroxidase (GPx) activity can cause CC. This study aims to show whether GPx Oxidized (GSSG)/ reduced (GSH) levels, which are considered oxidative markers, effective etiopathogenesis Erythrocyte isolation was performed 3 ml blood sample taken from volunteers aged 18-75 years. Hemoglobin amounts were determined standard graph drawn by monitoring conversion methemoglobin cyanmethemoglobin presence cyanide at 540 nm. Glutathione spectrophotometric NADPH+H+ (reduced nicotinamide adenine dinucleotide phosphate) oxidation a wavelength 340 The oxidized using following 412 nm formation 2-nitro-5-thiobenzoic acid, has yellow color. individuals with CC 5.64 ± 1.49 U/gHb, GSH concentration 6.96 1.45 nmol/gHb, GSH/GSSG ratio 1.04 0.49, healthy 10.52 2.22 11.43 1.90 GSH/GSSG: 3.86 1.30, is, values patient group significantly lower than control group. Current results suggest activity, be used markers diagnosis disease course, decrease these parameters may associated an increased risk